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| Status |
Public on Feb 23, 2020 |
| Title |
Increase in innate lymphoid cells during acute ST-segment elevated myocardial infarction and its clinical relevance |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to use RNA-seq to compare ILC1s gene expression in ST segmment elevation myocardial infarction patient compared with healthy control.
Results: Using an optimized data analysis workflow, More than 70 million clean reads were obtained from each sample group after elimination of low-quality reads. A total of 4204 DEGs were found up-regulated and 4712 DEGs down-regulated on comparison of ILC1s from STEMI patients and controls.
Conclusion: The dynamic number and gene expression changes in the subtype ILC1s indicate the role of ILCs in STEMI.
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| Overall design |
Methods: ILC1s mRNA profiles of STEMI patients and those of control were generated by deep sequencing, using Hiseq Xten . The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.
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| Contributor(s) |
Zhang M, Li J |
| Citation(s) |
32198366 |
| Submission date |
Mar 05, 2019 |
| Last update date |
Mar 30, 2020 |
| Contact name |
mingyou zhang |
| E-mail(s) |
mingyouz@me.com
|
| Phone |
13756896722
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| Organization name |
employer
|
| Street address |
519 dongminzhu
|
| City |
CHANGCHUN |
| State/province |
JILIN |
| ZIP/Postal code |
130021 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA525651 |
| SRA |
SRP187551 |
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