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Series GSE127996 Query DataSets for GSE127996
Status Public on Dec 31, 2019
Title Expression data from MCP-1-treated human kidney cancer CRL-1932 cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Microarray analysis revealed MCP-1 treatment altered protein folding processes in RCC CRL1932 cells. In response to MCP-1 treatment, CRL1932 cells and xenograft tumors expressed MCP-1-induced protein (MCPIP) which was reported to cause endoplasmic reticulum (ER) stress-induced apoptosis in human cardiomyocytes. In line with MCPIP induction, the expression of ER stress mediators, such as GRP78, PERK, IRE1α, and PDI, as well as molecules involved in ER stress-induced apoptosis, CHOP, calnexin, and Ero1α, presented in MCP-1 treated RCC cell line and xenograft tumors whereas absent or downregulated in untreated controls. TUNEL assay confirmed apoptosis of MCP-1 treated CRL1932 cells. MCPIP ectopically expressed in HEK293 cell resulted in apoptosis. Meta-analysis showed low level of MCP-1 associated with lower one year-survival rate after nephrectomy in RCC.
In this dataset, we include the expression array data from human kidney cancer CRL-1932 cell line with or without the treatment of MCP-1. These data were used to obtain genes upregulated in MCP-1-treated CRL-1932 cells.
 
Overall design In total, 4 samples were analyzed. We generated the following pairwise comparisons using Affymetrix Expression Console™ Software (version 1.4): CRL 1932 compared with MCP-1-CRL1932. Genes with a P value < 0.05 and a fold-change ≥1.5 were selected. To identify the potential epigenetic targets of MCP-1, we performed Venn diagram analysis with genes showing either up-regulated by MCP-1 treatment.
 
Contributor(s) Lee C, Sun C
Citation(s) 31816951
Submission date Mar 07, 2019
Last update date Dec 31, 2019
Contact name Chia Huei Lee
E-mail(s) chlee124@nhri.edu.tw
Organization name Taiwan National Health Research Institute
Department National Institute of Cancer Research
Street address 35, Keyan Rd.
City Zhunan Miaoli County
ZIP/Postal code 114
Country Taiwan
 
Platforms (1)
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (4)
GSM3659976 CRL1932-untreated-rep-1
GSM3659977 CRL1932-untreated-rep-2
GSM3659978 MCP-1-treated CRL 1932, bio rep-1
Relations
BioProject PRJNA526022

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Supplementary file Size Download File type/resource
GSE127996_RAW.tar 4.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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