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Series GSE127998 Query DataSets for GSE127998
Status Public on Mar 08, 2019
Title Genome-wide maps of Twist2-binding, MyoD-binding and chromatin state in Twist2-overexpressing Twist2+ cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Integrated analysis of genome-wide ChIP-Seq and RNA-Seq data revealed the first dynamic chromatin and transcriptional landscape of Twist2 binding during myogenic differentiation. During differentiation, Twist2 competes with MyoD at shared DNA motifs to direct global gene transcription and repression of the myogenic program. Additionally, TWIST2 shapes the epigenetic landscape to drive chromatin opening at oncogenic loci and chromatin closing at myogenic loci. These epigenetic changes redirect MyoD binding from myogenic genes towards oncogenic, metabolic, and growth genes.
 
Overall design ChIP-Seq of Twist2, MyoD, H3K27ac, and H3K27me3 in Twist2-overexpressing Twist2+ myoblasts
 
Contributor(s) Li S, Chen K, Barnes SD, Malladi VS, Xu L
Citation(s) 30975722
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AR071980 Role of Twist2+ myogenic progenitor cells in adult skeletal muscle UT SOUTHWESTERN MEDICAL CENTER Ning Liu
F30 AG056075 The Role of Twist2 in Regulating Myogenesis UT SOUTHWESTERN MEDICAL CENTER Stephen Li
R01 AR067294 Molecular Dissection of Myoblast Fusion In Muscle Development and Regeneration UT SOUTHWESTERN MEDICAL CENTER ERIC N Olson
R01 HL130253 Deciphering the role of a novel micropeptide in cardiac function and dysfunction UT SOUTHWESTERN MEDICAL CENTER ERIC N Olson
U54 HD087351 Myoediting of Duchenne muscular dystrophy UT SOUTHWESTERN MEDICAL CENTER ERIC N Olson
R01 HL138426 Chemically assisted remodeling of infarcted heart tissue by targeting Wnt lipidation UT SOUTHWESTERN MEDICAL CENTER RHONDA BASSEL-DUBY
Submission date Mar 07, 2019
Last update date Jun 07, 2019
Contact name Stephen Li
E-mail(s) stephen.li@utsouthwestern.edu
Organization name University of Texas Southwestern Medical Center
Department Molecular Biology
Lab Olson Lab
Street address 6000 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (34)
GSM3659984 GFP_GM_Input_1
GSM3659985 GFP_GM_Ty1_4
GSM3659986 GFP_GM_MyoD_2
Relations
BioProject PRJNA526023
SRA SRP187853

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127998_RAW.tar 6.1 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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