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Status |
Public on Mar 08, 2019 |
Title |
Genome-wide maps of Twist2-binding, MyoD-binding and chromatin state in Twist2-overexpressing Twist2+ cells |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Integrated analysis of genome-wide ChIP-Seq and RNA-Seq data revealed the first dynamic chromatin and transcriptional landscape of Twist2 binding during myogenic differentiation. During differentiation, Twist2 competes with MyoD at shared DNA motifs to direct global gene transcription and repression of the myogenic program. Additionally, TWIST2 shapes the epigenetic landscape to drive chromatin opening at oncogenic loci and chromatin closing at myogenic loci. These epigenetic changes redirect MyoD binding from myogenic genes towards oncogenic, metabolic, and growth genes.
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Overall design |
ChIP-Seq of Twist2, MyoD, H3K27ac, and H3K27me3 in Twist2-overexpressing Twist2+ myoblasts
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Contributor(s) |
Li S, Chen K, Barnes SD, Malladi VS, Xu L |
Citation(s) |
30975722 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 AR071980 |
Role of Twist2+ myogenic progenitor cells in adult skeletal muscle |
UT SOUTHWESTERN MEDICAL CENTER |
Ning Liu |
F30 AG056075 |
The Role of Twist2 in Regulating Myogenesis |
UT SOUTHWESTERN MEDICAL CENTER |
Stephen Li |
R01 AR067294 |
Molecular Dissection of Myoblast Fusion In Muscle Development and Regeneration |
UT SOUTHWESTERN MEDICAL CENTER |
ERIC N Olson |
R01 HL130253 |
Deciphering the role of a novel micropeptide in cardiac function and dysfunction |
UT SOUTHWESTERN MEDICAL CENTER |
ERIC N Olson |
U54 HD087351 |
Myoediting of Duchenne muscular dystrophy |
UT SOUTHWESTERN MEDICAL CENTER |
ERIC N Olson |
R01 HL138426 |
Chemically assisted remodeling of infarcted heart tissue by targeting Wnt lipidation |
UT SOUTHWESTERN MEDICAL CENTER |
RHONDA BASSEL-DUBY |
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Submission date |
Mar 07, 2019 |
Last update date |
Jun 07, 2019 |
Contact name |
Stephen Li |
E-mail(s) |
stephen.li@utsouthwestern.edu
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Organization name |
University of Texas Southwestern Medical Center
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Department |
Molecular Biology
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Lab |
Olson Lab
|
Street address |
6000 Harry Hines Blvd
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City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (34)
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Relations |
BioProject |
PRJNA526023 |
SRA |
SRP187853 |