|
| Status |
Public on Nov 07, 2019 |
| Title |
Loss of IDH1R132H mediated D-2HG production induces genome-wide demethylation in GCIMP low loci in patient-derived glioma cells |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Here, we describe and characterize CRISPR-Cas9 based isogenic cell line models using patient-derived IDH1R132H/WT glioma cell lines, and we use these models to explore changes in DNA methylation following genetic deletion of IDH1 alleles that ablate D2HG production.
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| |
|
| Overall design |
Comparison of CRISPR IDH1 targeted vs. parental cells or unedited clones methylation values.
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| |
|
| Contributor(s) |
Moure CJ, Chen LH, Yan H |
| Citation(s) |
31292202 |
| Submission date |
Mar 07, 2019 |
| Last update date |
Nov 09, 2019 |
| Contact name |
Hai Yan |
| E-mail(s) |
hai.yan@dm.duke.edu
|
| Organization name |
Duke University
|
| Department |
Pathology
|
| Lab |
Yan Lab
|
| Street address |
199B-MSRB, Research Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA526085 |
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