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Series GSE12826 Query DataSets for GSE12826
Status Public on Sep 18, 2008
Title Zebrafish 24hpf mutant sk8 gene expression
Organism Danio rerio
Experiment type Expression profiling by array
Summary The production of functional mRNA involves multiple steps including transcription initiation, elongation, and termination. spt5 encodes a conserved essential transcription elongation factor that controls RNAPII processivity in vitro and co-localizes with RNAPII in vivo.
We used microarrays to detail the global expression of gene expression at 24 hours post fertilization in wild-type and Spt5 mutant (fog-sk8) to identify Spt5 transcriptionally dependent genes and affected pathways. Here we report that a mutation in zebrafish spt5, sk8, deregulates <5% of ~10,000 genes examined. These findings reveal a small but diverse set of developmentally regulated genes, whose expression is critically dependent on Spt5-regulated RNAPII stalling in vertebrate development.

Keywords: comparison
 
Overall design RNA from zebrafish embryos were harvested at 24 hours post fertilization for hybridization to Affymetrix gene arrays.
 
Contributor(s) Su G, Keerthi K, Nathan S
Citation(s) 18978947
Submission date Sep 17, 2008
Last update date Jan 25, 2018
Contact name Nathan Salomonis
E-mail(s) nathan.salomonis@cchmc.org
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM322065 Zebrafish_wt_24hpf, biological rep1
GSM322066 Zebrafish_wt_24hpf, biological rep2
GSM322067 Zebrafish_wt_24hpf, biological rep3
Relations
BioProject PRJNA111131

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12826_RAW.tar 11.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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