|
| Status |
Public on Sep 18, 2008 |
| Title |
Zebrafish 24hpf mutant sk8 gene expression |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
The production of functional mRNA involves multiple steps including transcription initiation, elongation, and termination. spt5 encodes a conserved essential transcription elongation factor that controls RNAPII processivity in vitro and co-localizes with RNAPII in vivo.
We used microarrays to detail the global expression of gene expression at 24 hours post fertilization in wild-type and Spt5 mutant (fog-sk8) to identify Spt5 transcriptionally dependent genes and affected pathways. Here we report that a mutation in zebrafish spt5, sk8, deregulates <5% of ~10,000 genes examined. These findings reveal a small but diverse set of developmentally regulated genes, whose expression is critically dependent on Spt5-regulated RNAPII stalling in vertebrate development.
Keywords: comparison
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| |
|
| Overall design |
RNA from zebrafish embryos were harvested at 24 hours post fertilization for hybridization to Affymetrix gene arrays.
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| |
|
| Contributor(s) |
Su G, Keerthi K, Nathan S |
| Citation(s) |
18978947 |
| Submission date |
Sep 17, 2008 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
|
| Samples (6)
|
| GSM322065 |
Zebrafish_wt_24hpf, biological rep1 |
| GSM322066 |
Zebrafish_wt_24hpf, biological rep2 |
| GSM322067 |
Zebrafish_wt_24hpf, biological rep3 |
| GSM322068 |
Zebrafish_mutant_sk8_24hpf, biological rep1 |
| GSM322069 |
Zebrafish_mutant_sk8_24hpf, biological rep2 |
| GSM322070 |
Zebrafish_mutant_sk8_24hpf, biological rep3 |
|
| Relations |
| BioProject |
PRJNA111131 |
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