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| Status |
Public on Mar 14, 2019 |
| Title |
mRNA regions where 80S ribosomes pause during translation elongation in vivo interact with protein uS19, a component of the decoding site |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
80S-ribosome bound uS19 translatome-wide crosslinking sites in human mRNAs revealed by PAR-CLIP
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| Overall design |
We focused on determining the uS19 contacts with mRNAs in vivo by applying the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method to a stable HEK293 cell line inducibly producing FLAG-tagged uS19 and grown on a medium containing 4-thiouridine.
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| |
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| Contributor(s) |
Babaylova ES, Gopanenko AV, Bulygin KN, Tupikin AE, Kabilov MR, Malygin AA, Karpova GG |
| Citation(s) |
31802126 |
| Submission date |
Mar 13, 2019 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Marsel Kabilov |
| E-mail(s) |
kabilov@niboch.nsc.ru
|
| Organization name |
ICBFM SB RAS
|
| Lab |
Genomics Core Facility
|
| Street address |
Lavrentyev av. 8
|
| City |
Novosibirsk |
| ZIP/Postal code |
630090 |
| Country |
Russia |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA526909 |
| SRA |
SRP188365 |
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