|
| Status |
Public on Feb 14, 2020 |
| Title |
Identification and characterization of artery and vein enhancers in the human genome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
Our goal was to identify artery- and vein-specific cis regulatory elements in the human genome and use these to determine novel mechanisms that regulate arteriovenous differentiation
|
| |
|
| Overall design |
Artery and vein enhancers were identified using ChIP-Seq for K27ac and EP300 on HUAEC and HUVEC, performed in duplicate. Further analysis included RNAseq in triplicate from the same cell types. To assess binding of known transcription factors NR2F2 and ERG, we performed ChIP-Seq in duplicate samples. We assessed K27ac and K27me3 occupancy under different experimental conditions using triplicate samples from HUVEC
|
| |
|
| Contributor(s) |
Lawson N |
| Citation(s) |
32065070 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 HL140017 |
Embryonic origins of endothelial heterogeneity |
UNIV OF MASSACHUSETTS MED SCHOOL |
NATHAN D LAWSON |
|
| Submission date |
Mar 15, 2019 |
| Last update date |
May 15, 2020 |
| Contact name |
Nathan D. Lawson |
| E-mail(s) |
nathan.lawson@umassmed.edu
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell, and Cancer Biology
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (58)
|
|
| Relations |
| BioProject |
PRJNA527296 |
| SRA |
SRP188557 |
Less...
More...