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Series GSE12852 Query DataSets for GSE12852
Status Public on Sep 20, 2008
Title Gene expression profile in pelvic organ prolapse
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Introduction and Hypothesis: Identify processes contributing to pelvic organ prolapse (POP) by transcriptional profiling of pelvic connective tissue in conjunction with light microscopy. Methods: We performed a frequency matched case-control study of women undergoing hysterectomy. Total RNA, extracted from uterosacral and round ligament samples used to generate labeled cRNA, was hybridized to microarrays and analyzed for the expression of 32,878 genes. Significance Analysis of Microarrays, (Stanford University, CA), identified differentially expressed genes used for ontoanalysis, and quantitative PCR (qPCR) confirmed results. Light microscopy confirmed tissue type and assessed inflammatory infiltration. Results: The analysis of thirty-four arrays revealed 249 differentially expressed genes with fold changes larger than 1.5 fold and false discovery rates ≤5.2%. Immunity and Defense was the most significant biological process differentially expressed in POP. Selected qPCR confirmed 4 genes. Light microscopy showed no inflammatory infiltrates. Conclusions: Genes enriched for Immunity and Defense contribute to POP independent of inflammatory infiltrates.

Keywords: whole tissue (endopelvic fascia) type comparison
 
Overall design This was a group matched case control study of 8 women with pelvic organ prolapse versus 9 non-prolapse controls, both undergoing hysterectomy for benign conditions. Two separate pelvic support tissues were collected from each patient. The uterosacral ligament and round ligament tissue was removed at the time of hysterectomy, RNA was extracted and ABI whole genome chips used to identify differences in expression profiles of individual samples. Various ethnic groups, age groups and menopausal status were included.
 
Contributor(s) Brizzolara SS, Urschitz J, Killeen J
Citation(s) 19056808
Submission date Sep 19, 2008
Last update date Jan 27, 2016
Contact name Shawna S Brizzolara
E-mail(s) brizzola@hawaii.edu
Phone 808 203-6539
Fax 808 955-2174
Organization name Shawna Brizzolara, MD
Department Obstetrics and Gynecology
Lab John A burns School of Mecicine
Street address 1319 Punahou St #824
City Honolulu
State/province HI
ZIP/Postal code 96826
Country USA
 
Platforms (1)
GPL2986 ABI Human Genome Survey Microarray Version 2
Samples (34)
GSM322499 round ligament_POP_premenopause_subject1
GSM322500 round ligament_POP_premenopause_subject7
GSM322501 round ligament_POP_premenopause_subject10
Relations
BioProject PRJNA111083

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12852_RAW.tar 62.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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