|
| Status |
Public on Feb 20, 2020 |
| Title |
DNA hydroxymethylation profiling of paired parental and therapy resistant cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
Genome wide DNA methylation profiling of paired parental and therapy resistant cancer cell lines. Parental cell lines are mostly established cell lines. Resistant cell lines were obtained through long term exposure of the parental cells to gradually increasing doses of cancer therapies. Followig the published TAB-sequencing protocol, the Illumina Infinium 450K or EPIC Human DNA methylation Beadchips were used to obtain DNA hydroxymethylation profiles across approximately 450,000 or 850,000 CpGs from the cells. Samples include 5 parental and 6 derived resistant cell lines.
|
| |
|
| Overall design |
DNA from the 11 samples processed according to the TAB-seq protocol were hybridised to the Illumina Infinium 450K or EPIC Human Methylation Beadchips
|
| |
|
| Contributor(s) |
Galle E, Lambrechts D |
| Citation(s) |
32059745 |
| Submission date |
Mar 21, 2019 |
| Last update date |
Feb 24, 2020 |
| Contact name |
Diether Lambrechts |
| E-mail(s) |
diether.lambrechts@kuleuven.be
|
| Organization name |
KU Leuven
|
| Department |
Human Genetics
|
| Lab |
Laboratory for Translational Genetics
|
| Street address |
Herestraat 49
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
| |
|
| Platforms (2) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
| GPL21145 |
Infinium MethylationEPIC |
|
| Samples (11)
|
| GSM3682539 |
genomic DNA from HepG2_hydroxymethylation |
| GSM3682540 |
genomic DNA from HepG2S1_hydroxymethylation |
| GSM3682541 |
genomic DNA from HepG2S3_hydroxymethylation |
| GSM3682542 |
genomic DNA from PancVB_hydroxymethylation |
| GSM3682543 |
genomic DNA from PancB1Q_hydroxymethylation |
| GSM3682544 |
genomic DNA from HCC4006P1_hydroxymethylation |
| GSM3682545 |
genomic DNA from HCC4006ER_hydroxymethylation |
| GSM3682546 |
genomic DNA from HCC4006P2_hydroxymethylation |
| GSM3682547 |
genomic DNA from HCC4006GR_hydroxymethylation |
| GSM3682548 |
genomic DNA from HCC827P_hydroxymethylation |
| GSM3682549 |
genomic DNA from HCC827GR_hydroxymethylation |
|
| This SubSeries is part of SuperSeries: |
| GSE128686 |
RNA-seq and DNA methylation and hydroxymethylation profiles in sensitive and treatment-resistant cancer cell lines |
|
| Relations |
| BioProject |
PRJNA528493 |
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