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Series GSE128804 Query DataSets for GSE128804
Status Public on Jan 29, 2020
Title Serine racemase is required for colorectal tumor growth and a potential therapeutic target.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Serine racemase (SRR) is an enzyme that catalyzes two reactions, the racemization and the dehydration of L-Ser and D-Ser and has been studied mainly in the fields of central nervous system. The function of SRR in cancer metabolism has not been elucidated. Here we show that SRR expression is upregulated in colorectal adenoma and adenocarcinoma lesions compared to non-neoplastic mucosa in human colorectal cancer specimens. Further analysis by using CRISPR/CRISPR-associated protein 9 system reveals that SRR enhances colorectal cancer cell proliferation via maintaining intracellular pyruvate and mitochondrial mass resulting in higher basal reactive oxygen species level, and has anti-apoptotic function.
 
Overall design To investigate the effects of SRR on gene expression, we performed RNA-seq analysis and found 1,571 genes downregulated in SRR-KO DLD-1 cells and 977 genes in SRR-KO HCT116 cells.
Web link https://0-www-nature-com.brum.beds.ac.uk/articles/s42255-019-0156-2
 
Contributor(s) Ohshima K, Okuzaki D, Morii E
Citation(s) 32694681
Submission date Mar 25, 2019
Last update date Jul 26, 2020
Contact name Daisuke Okuzaki
E-mail(s) dokuzaki@biken.osaka-u.ac.jp
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM3685744 SRR-KO of HCT-116 cells
GSM3685745 EV cells of HCT-116 cells
GSM3685746 SRR-KO of DLD-1 cells
Relations
BioProject PRJNA528919
SRA SRP189346

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128804_RAW.tar 520.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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