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GEO help: Mouse over screen elements for information. |
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Status |
Public on Sep 06, 2019 |
Title |
RNA-Seq analysis miR-455-3p overexpression effect on breast cancer cell line |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
MiR-455-3p has been reported to suppress the expression of ROCK2, HOXB5, hTERT, and RUNX2, which are involved in multiple biological processes including renal fibrosis, chondrogenic development and differentiation, Alzheimer’s disease, preeclampsia, and tumorigenesis. Here, we showed that miR-455-3p broadly inhibited TGF-beta-induced EMT by repressing the expression of Smad2, ZEB1, and HDAC2 in breast cancer cells, and that miR-455-3p loss-of-function promotes the acquisition of cell invasive properties and the ability to metastasize. These results indicate that miR-455-3p represents an important node that inhibits metastasis of breast cancer.
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Overall design |
MiR-455-3p mimics or controls were transfected into MCF-7 cells (n=3 for each group) for 48 hours. Total RNA was isolated using Trizol reagent (Invitrogen, USA). Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.
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Contributor(s) |
Zeng Y, Wang Y |
Citation(s) |
31492753 |
Submission date |
Apr 01, 2019 |
Last update date |
Dec 17, 2019 |
Contact name |
Yan Wang |
E-mail(s) |
yanwang@tmu.edu.cn
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Organization name |
Tianjin Medical University
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Department |
Department of Biochemistry and Molecular Biology
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Street address |
#22 Qixiangtai Road
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City |
Tianjin |
ZIP/Postal code |
300070 |
Country |
China |
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Platforms (1) |
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Samples (6)
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Relations |
BioProject |
PRJNA530241 |
SRA |
SRP190040 |
Supplementary file |
Size |
Download |
File type/resource |
GSE129146_RAW.tar |
1.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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