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Series GSE129146 Query DataSets for GSE129146
Status Public on Sep 06, 2019
Title RNA-Seq analysis miR-455-3p overexpression effect on breast cancer cell line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary MiR-455-3p has been reported to suppress the expression of ROCK2, HOXB5, hTERT, and RUNX2, which are involved in multiple biological processes including renal fibrosis, chondrogenic development and differentiation, Alzheimer’s disease, preeclampsia, and tumorigenesis. Here, we showed that miR-455-3p broadly inhibited TGF-beta-induced EMT by repressing the expression of Smad2, ZEB1, and HDAC2 in breast cancer cells, and that miR-455-3p loss-of-function promotes the acquisition of cell invasive properties and the ability to metastasize. These results indicate that miR-455-3p represents an important node that inhibits metastasis of breast cancer.
 
Overall design MiR-455-3p mimics or controls were transfected into MCF-7 cells (n=3 for each group) for 48 hours. Total RNA was isolated using Trizol reagent (Invitrogen, USA). Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.
 
Contributor(s) Zeng Y, Wang Y
Citation(s) 31492753
Submission date Apr 01, 2019
Last update date Dec 17, 2019
Contact name Yan Wang
E-mail(s) yanwang@tmu.edu.cn
Organization name Tianjin Medical University
Department Department of Biochemistry and Molecular Biology
Street address #22 Qixiangtai Road
City Tianjin
ZIP/Postal code 300070
Country China
 
Platforms (1)
GPL23227 BGISEQ-500 (Homo sapiens)
Samples (6)
GSM3701260 Control rep1
GSM3701261 Control rep2
GSM3701262 Control rep3
Relations
BioProject PRJNA530241
SRA SRP190040

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129146_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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