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| Status |
Public on Apr 02, 2019 |
| Title |
Transcriptome analysis of lung fibroblasts from 5 control and 5 IPF patients with/out TGF-b1 stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Isolated primary human fibroblasts from 5 control and 5 IPF patients were treated with/out 3ng/ml TGF-b1 for 24 hours before cells were lysed for RNA extraction.
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| |
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| Overall design |
A total of 20 samples were processed for RNA extraction including an on-column Dnase treatment. RNA quantity and quality were measured (RIN values >8.0) before samples were processed according to the Affymetrix GeneChip Human Transcriptome Arrays 2.0 user manual.
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| |
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| Contributor(s) |
Hadjicharalambous MR, Roux BT, Csomor E, Feghali-Bostwick CA, Murray LA, Clarke DL, Lindsay MA |
| Citation(s) |
30988425 |
| Submission date |
Apr 01, 2019 |
| Last update date |
Jul 11, 2019 |
| Contact name |
Marina Hadjicharalambous |
| E-mail(s) |
mh939@bath.ac.uk
|
| Organization name |
University of Bath
|
| Street address |
PHARMACY AND PHARMACOLOGY (5 WEST), CLAVERTON DOWN
|
| City |
BATH |
| ZIP/Postal code |
BA2 7AY |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (20)
|
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| Relations |
| BioProject |
PRJNA530261 |
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