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| Status |
Public on Apr 17, 2019 |
| Title |
Interleukin-17 receptor D constitutes an alternative receptor for interleukin-17A important in psoriasis-like skin inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the overall function of IL-17RD and IL-17RC in IL-17A signaling, RNAseq was performed with WT, Il17rc KO, and Il17rd KO primary mouse keratinocytes following IL-17A treatment along with untreated WT control. Keratinocyte from neonatal WT, Il17rd KO, and Il17rc KO mice were cultured and stimulated with IL-17A (100 ng/mL, PeproTech) for 8h.
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| Overall design |
Keratinocyte from neonatal WT, Il17rd KO, and Il17rc KO mice were cultured in 154CF medium plus HKGS kit. Cells were stimulated with IL-17A (100 ng/mL) for 8hm and then harvest for RNA-seq.
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| Contributor(s) |
Su Y, Zhao X |
| Citation(s) |
31175175 |
| Submission date |
Apr 02, 2019 |
| Last update date |
Jul 22, 2019 |
| Contact name |
Chen Dong |
| Organization name |
Tsinghua University
|
| Department |
Institute for Immunology and School of Medicine
|
| Lab |
Dongchen's lab
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA530390 |
| SRA |
SRP190124 |
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