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| Status |
Public on Sep 11, 2019 |
| Title |
Expression data from splenic IL-10 positive and IL-10 negative B cells of C57BL/6 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The mechanisms in regulating the development of IL-10 producing B cells are largely unknown. To clarify which transcription factor is critical for regulation of IL-10 producing B cells, we compared the gene expression profile of IL-10 positive B cells and IL-10 negative B cells. Transcriptional repressor Prdm1/Blimp1 is known to play a key role in controlling B ells differentiation. We show a dual role for Blimp-1 in IL-10 expression in IL-10 producing B cells and plasmablasts.
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| Overall design |
To understand the genes involved in regulating the production of Il-10 by B cells, purified splenic B cells were stimulated with LPS or anti-CD40 for 48 hrs, followed by isolation of IL-10+ B cells and IL-10− B cells.
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| Contributor(s) |
Wang Y, Lin I, Lin K |
| Citation(s) |
31474988 |
| Submission date |
Apr 03, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Ying-Hsiu Wang |
| E-mail(s) |
bi.bi.hsiu@gmail.com
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| Organization name |
National Defense Medical Center
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| Street address |
No.161, Sec. 6, Minquan E. Rd., Neihu Dist.
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| City |
Taipei City |
| ZIP/Postal code |
11490 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| GSM3703675 |
IL-10 positive B cells, anti-CD40 for 48 h, biological rep1 |
| GSM3703676 |
IL-10 negative B cells, anti-CD40 for 48 h, biological rep1 |
| GSM3703677 |
IL-10 positive B cells, LPS for 48 h, biological rep1 |
| GSM3703678 |
IL-10 negative B cells, LPS for 48 h, biological rep1 |
| GSM3703679 |
IL-10 positive B cells, anti-CD40 for 48 h, biological rep2 |
| GSM3703680 |
IL-10 negative B cells, anti-CD40 for 48 h, biological rep2 |
| GSM3703681 |
IL-10 positive B cells, LPS for 48 h, biological rep2 |
| GSM3703682 |
IL-10 negative B cells, LPS for 48 h, biological rep2 |
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| Relations |
| BioProject |
PRJNA530683 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE129260_All_entities.xlsx |
10.9 Mb |
(ftp)(http) |
XLSX |
| GSE129260_RAW.tar |
24.8 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE129260_f1.8.xlsx |
29.4 Kb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data are available on Series record |
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