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| Status |
Public on Nov 02, 2020 |
| Title |
NG Capture-C from human erythroid and embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
NG Capture-C provides high resolution chromatin conformation capture (3C) interaction maps. NG Capture-C was carried out in erythroid and hESC cells at the Hba, Hbb, Myc, and Slc25a37 locus to generate sequence libraries for analytical software testing.
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| Overall design |
NG Capture-C was performed in Day 10 differentiating erythroid (Ery) cells from three donors, and human Embryonic Stem Cells (H1 hESC; WiCell) from four independently grown cultures. Capture-C was performed with biotinylated oligonucleotides targeting sequences adjacent to DpnII sites in targeted to the Hba-1, Hba-2, Hbb, Hbd, Myc and Slc25a37 promoters.
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| |
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| Contributor(s) |
Downes DJ, Hughes JR |
| Citation(s) |
33483495 |
| Submission date |
Apr 05, 2019 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Damien Downes |
| E-mail(s) |
damien.downes@ndcls.ox.ac.uk
|
| Phone |
01865222374
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| Organization name |
The University of Oxford
|
| Department |
MRC Weatherall Institute of Medicine
|
| Street address |
John Radcliffe Hospital
|
| City |
Headington |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA531102 |
| SRA |
SRP191038 |
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