NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE129506 Query DataSets for GSE129506
Status Public on Sep 20, 2019
Title HIV infection and use of illicit substances promote secretion of semen exosomes that reprogram monocyte morphology
Organism Homo sapiens
Experiment type Expression profiling by array
Summary HIV transmission involves the adhesion to and migration of infected cells, including monocytes across biological barriers comprising extracellular matrix components. Recently, semen exosomes (SE) from HIV uninfected (HIV-) and HIV infected (HIV+) subjects were shown to contain molecules that were adhesive to Jurkat T cells. Since monocyte trafficking across various biological barriers are intensified by HIV and/or illicit substances, such as cocaine, we sought to understand how SE from HIV infected subjects’ comorbid with illicit drug abuse influence monocyte gene expression and behavior. Therefore, a model of U937 monocyte adhesion on collagen-coated surface in the presence and absence of SE from various clinical (HIV-Drug-, HIV-Drug+, HIV+Drug-, and HIV+Drug+) groups was developed. Using this system, we demonstrate by Microarray analysis that SE from different clinical groups reprogramed gene expression profile of monocytes cultured atop collagen.
In line with altered gene expression is increased adhesion of monocytes to collagen following treatment with SE from all clinical groups compared to vehicle. Interestingly, SE from HIV+ subjects’ comorbid with drug abuse potentiated monocyte adhesion to collagen. Mechanistically, SE-Drug and SE-HIV altered monocyte shape, induced actin cytoskeleton reorganization, formation of membrane ruffles and lamellipodia-like structures, as well as focal-adhesion contacts. The cytoskeletal reorganization induced by SE-Drug is associated with impairment of cell motility, while SE-HIV increased cell chemotaxis toward HIV secretome. The observation of SE-Drug and SE-HIV mediated alteration of monocyte transcriptome and cellular behavior reported herein is key to understanding the contribution of exosomes to long-term neuro, vascular, and mucosal perturbations associated with psychostimulants (methamphetamine, cocaine, opioids, and alcohol) comorbidity with HIV infection.
 
Overall design Three Biological replicates of U937 cells were treated with vehicle PBS (P) or with semen exosomes from one of the four different clinical groups (A: HIV-Drug-, B: HIV-Drug+, C: HIV+Drug-, and D: HIV+Drug+)
 
Contributor(s) Lyu Y, Kaddour H, Kopcho S, Panzner T, Shouman N, Okeoma cM
Citation(s) 31484431
Submission date Apr 09, 2019
Last update date Nov 04, 2019
Contact name Hussein Kaddour
E-mail(s) hussein.kaddour@stonybrook.edu
Phone 2344141414
Organization name Stony Brook University
Department Pharmacological Sciences
Lab Okeoma - BST 8-120
Street address 100 Nicolls Road
City Stony Brook
State/province NY
ZIP/Postal code 11794
Country USA
 
Platforms (1)
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (15)
GSM3714526 U937-collagen_PBS_rep1
GSM3714527 U937-collagen_PBS_rep2
GSM3714528 U937-collagen_PBS_rep3
Relations
BioProject PRJNA531605

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129506_RAW.tar 17.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap