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Series GSE1296 Query DataSets for GSE1296
Status Public on Apr 09, 2004
Title Simple ambient preservation of human tissues for RNA expression array analysis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used quantitative RNA expression profiling on the Affymetrix U133 human expression array to validate quantitative expression results obtained with the tissue preservative RNALater against snap frozen and fresh tissues as a means of routine tissue collection and temporary storage. By using split samples from a homogenous tissue (uterine myometrium), and including duplicates within each processing group compared, we were able to undertake a formal ANOVA analysis comparing the magnitude of result variation contributed by sample source (different patients), processing protocol (fresh vs. frozen vs. 24 or 72 hours RNALater), and random background (duplicates). The dataset was randomly permuted to define a baseline pattern of test statistic values against which the observed results could be interpreted. Ambient storage of tissues for 24 or 72 hours in RNALater did not contribute any systematic shift in quantitative RNA expression results relative to the alternatives of fresh or frozen tissue.
Keywords: parallel sample
 
 
Citation(s) 15537428
Submission date Apr 07, 2004
Last update date Aug 10, 2018
Contact name Chunmei Liu
E-mail(s) chunmei_liu@affymetrix.com
Phone 408 7315502
Fax 4084810422
URL http://www.affymetrix.com
Organization name Affymetrix
Department
Lab Clinical Genomics R & D
Street address 3380 Central Expressway
City Santa Clara
State/province CA
ZIP/Postal code 95051
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (24)
GSM21179 uterine myometrium 02-03 D1b
GSM21180 uterine myometrium 02-02 D1a 24hrs RNAlater
GSM21181 uterine myometrium 02-03 D3a 72hrs RNAlater
Relations
BioProject PRJNA90217

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