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Series GSE12991 Query DataSets for GSE12991
Status Public on Dec 25, 2008
Title Isolation of single miRNA-expressing cells from zebrafish embryos
Organism Danio rerio
Experiment type Expression profiling by array
Summary The goal of the project was to isolate single miRNA-expressing cells labelled by GFP reporter genes under the control of endogenous miRNA promoters and analyze expression levels of miRNA target genes in these cells. GFP-positive miRNA-expressing cells and GFP-negative cells from the rest of the embryos were purified at the same developmental stage to the cellular resolution using fluorescent activated cell sorting (FACS). Focus was on regulation by miR-206 and miR-133 in the developing somites and miR-124 in the developing central nervous system. Comparison of wild-type embryos and those lacking miRNAs revealed predicted
targets that responded to the miRNAs and distinguished miRNA-mediated mRNA
destabilization from other regulatory effects.
Keywords: cell type comparison
 
Overall design Using FACS, GFP-negative and GFP-positive cells were isolated from two different miRNA reporter wild-type lines as well as from a Dicer-mutant zebrafish line at the same developmental stage (14-16 somites). Three biological replicates for each condition were used for microarray analyses.
 
Citation(s) 19240133
Submission date Sep 30, 2008
Last update date Jan 25, 2018
Contact name Alena Shkumatava
E-mail(s) shkumata@wi.mit.edu
Organization name Whitehead Institute/MIT
Department Bartel Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (18)
GSM325510 wt_206_GFP_pos1, biological replicate 1
GSM325511 wt_206_GFP_neg1, biological replicate 1
GSM325512 wt_206_GFP_pos2, biological replicate 2
Relations
BioProject PRJNA112601

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12991_RAW.tar 39.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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