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| Status |
Public on Apr 19, 2019 |
| Title |
Induction and maintenance of specific multipotent progenitor stem cells synergistically mediated by Activin A and BMP4 signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We recently reported that epiblast stem cells (EpiSCs)-like cells could be derived from preimplantation embryos (named as AFSCs). Here, we established AFSCs from pre‐implantation embryos of multiple mouse strains and showed that unlike EpiSCs, the derivation efficiency of AFSCs was affected by the genetic background. We then used AFSCs lines to dissect the roles of Activin A (Act A) and basic fibroblast growth factor and reported that Act A alone was capable of maintaining self renewal but not developmental potential in vivo. Finally, we established a novel experimental system, in which AFSCs were efficiently converted to multipotent progenitor stem cells using Act A and BMP4 (named as ABSCs). Importantly, these ABSCs contributed to neural mesodermal progenitors and lateral plate mesoderm in postimplantation chimeras. Taken together, our study established a robust experimental system for
the generation of specific multipotent progenitor stem cells that was self-renewable and capable of contributing to embryonic and extra-embryonic tissues.
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| Overall design |
mRNA profiles of 3 cell types, generated by RNA sequencing in 3 duplicates, using HiSeq x10. Paired-end 150-bp sequencing was applied
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| Contributor(s) |
Chen Y, Bao S |
| Citation(s) |
32324269 |
| BioProject |
PRJNA532991 |
| Submission date |
Apr 18, 2019 |
| Last update date |
May 21, 2020 |
| Contact name |
Chen Yanglin |
| E-mail(s) |
21508011@mail.imu.edu.cn
|
| Organization name |
Southern Medical University
|
| Street address |
shatai road
|
| City |
Guangzhou |
| ZIP/Postal code |
510515 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| SRA |
SRP192724 |
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