|
| Status |
Public on Oct 02, 2020 |
| Title |
Intrahepatic lipocalin 2 promotes liver fibrosis in alcoholic hepatitis [array] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Our project is to explore the molecular subtypes for targeted therapies in Alcoholic Hepatitis (AH). We have found that LCN2 gene expression is markedly induced in AH patients and correlated to the disease severity including portal hypertension. Animal data from microarray experiments show that compared with the wild mice, LCN2 knockout mice are protected from CCl4-induced liver fibrosis. The focus of our current study is to investigate the effect of overexpression of LCN2 on functional changes in hepatocytes. To this end, HepG2 cells and human primary hepatocytes (HPH) were overexpressed human LCN2 gene and samples were analyzed through RNA-sequencing.
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| |
|
| Overall design |
To dissect the molecular mechanisms that mediate LCN2 fibrogenic effects in the injured liver, we performed transcriptome profiling analysis of Lcn2–/– and WT mice treated with CCl4.
Liver tissue was collected from BALB/c WT and Lcn2-/- mice treated with CCl4 or corn oil for 4 weeks.
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| |
|
| Citation(s) |
32968110 |
| Submission date |
Apr 22, 2019 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Ramon Bataller |
| E-mail(s) |
bataller@pitt.edu
|
| Organization name |
Pittsburgh Liver Research Center
|
| Street address |
200 Lothrop St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (16)
|
| GSM3732508 |
Wild type mice control replicate 4 |
| GSM3732509 |
Lcn2 knockout mice control replicate 1 |
| GSM3732510 |
Lcn2 knockout mice control replicate 2 |
| GSM3732511 |
Lcn2 knockout mice control replicate 3 |
| GSM3732512 |
Wild type mice CCl4-treated replicate 1 |
| GSM3732513 |
Wild type mice CCl4-treated replicate 2 |
| GSM3732514 |
Wild type mice CCl4-treated replicate 3 |
| GSM3732515 |
Wild type mice CCl4-treated replicate 4 |
| GSM3732516 |
Wild type mice CCl4-treated replicate 5 |
| GSM3732517 |
Lcn2 knockout mice CCl4-treated replicate 1 |
| GSM3732518 |
Lcn2 knockout mice CCl4-treated replicate 2 |
| GSM3732519 |
Lcn2 knockout mice CCl4-treated replicate 3 |
| GSM3732520 |
Lcn2 knockout mice CCl4-treated replicate 4 |
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| This SubSeries is part of SuperSeries: |
| GSE130129 |
Intrahepatic lipocalin 2 promotes liver fibrosis in alcoholic hepatitis |
|
| Relations |
| BioProject |
PRJNA534033 |
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