|
| Status |
Public on Apr 24, 2019 |
| Title |
Myeloid cells in liver and bone marrow acquire a functionally distinct inflammatory phenotype during obesity-related steatohepatitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Objective: Bone marrow-derived myeloid cells accumulate in the liver as monocytes and macrophages during the progression of obesity-related non-alcoholic fatty liver disease (NAFLD) to steatohepatitis (NASH). Myeloid cells comprise heterogeneous subsets, and dietary overnutrition may affect macrophages in liver and bone marrow. We therefore aimed at characterizing in-depth the functional adaptations of myeloid cells in fatty liver.
Design: We employed single-cell RNA-sequencing to comprehensively assess the heterogeneity of myeloid cells in liver and bone marrow during NAFLD, by analyzing C57BL/6 mice fed with a high-fat, high-sugar, high-cholesterol "Western diet" for 16 weeks. We also characterized NAFLD-driven functional adaptations of macrophages in vitro and their functional relevance during steatohepatitis in vivo.
|
| |
|
| Overall design |
Liver myeloid leukocytes and their bone marrow precursors adapt a common and functionally relevant inflammatory signature during NAFLD progression.
Bone marrow derived macrophages were cultivated for 7 days followed by 100 ng/ml LPS stimulation. Macrophages were preincubated for the first 4 days with and without 10 mM fatty acid (palmitic and oleic acid 1:3). ND=standard chow diet and WD=Western diet.
|
| |
|
| Contributor(s) |
Krenkel O, Hundertmark J, Abdallah AT, Kohlhepp M, Puengel T, Roth T, Branco DP, Mossanen JC, Luedde T, Trautwein C, Costa IG, Tacke F |
| Citation(s) |
31076404 |
| Submission date |
Apr 23, 2019 |
| Last update date |
Jun 07, 2019 |
| Contact name |
Oliver Krenkel |
| Organization name |
University Hospital Aachen
|
| Street address |
Pauwels Str. 30
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platforms (1) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA534228 |
| SRA |
SRP193451 |
Less...
More...