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| Status |
Public on Apr 26, 2019 |
| Title |
Microarray analysis of the effect of shRNA-mediated HAVCR2 knock-down (KD) in TIM-3-positive AML cell line, KASUMI-3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Purpose: To clarify the function of TIM-3 signaling in AML cells.
Analysis of lentiviral shRNA-mediated knockdown of TIM-3 in TIM-3+ acute myeloid leukemia (AML) cell line, KASUMI-3. Consistent with our previous study (Kikushige et al., Cell Stem Cell 2015) revealing the presence of TIM-3/galectin-9 autocrine loop and its constitutive signaling in AML, KD of receptor,TIM-3, alone significantly impaired the proliferation of KASUMI-3 in vitro and in vivo.
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| Overall design |
To cralify the effect of TIM-3 signaling in AML cells, we compared transcriptome datas of TIM-3 KD KASUMI-3 cells by 2 kinds of shRNA with those of scrambled control KASUMI-3 cells. Microarray experiment was performed with duplicate samples.
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| Contributor(s) |
Sakoda T, Kikushige Y |
| Citation(s) |
36745103 |
| Submission date |
Apr 25, 2019 |
| Last update date |
Aug 10, 2023 |
| Contact name |
Teppei Sakoda |
| E-mail(s) |
s_teppei@mti.biglobe.ne.jp
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| Organization name |
Kyushu University
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| Street address |
3-1-1, Maidashi, Higashi-ku
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| City |
Fukuoka |
| ZIP/Postal code |
8128582 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA535504 |
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