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| Status |
Public on Apr 30, 2019 |
| Title |
The transcriptomic response to albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), oxfendazole (OxBZ) and fenbendazole (FBZ) in the C. elegans strain CB3474 ben-1(e1880)III |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have undertaken a detailed analysis of the biotransformation of five of the most therapeutically important benzimidazole anthelmintics - albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), oxfendazole (OxBZ) and fenbendazole (FBZ) - in Caenorhabditis elegans. As a step to identifying nematode enzymes potentially responsible for benzimidazole biotransformation, we characterised the transcriptomic response to each of the benzimidazole drugs using the C. elegans strain CB3474 ben-1(e1880)III in order to minimize general phenotypic and stress responses to the drug. In the case of albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), and oxfendazole (OxBZ) - the shared transcriptomic response was dominated by the up- regulation classical xenobiotic response genes.
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| Overall design |
Three biological replicates of approximately 20,000 larvae C. elegans CB3474 ben-1(e1880)III exposed to 1.13mM of either albendazole (Sigma Cat# A4673), mebendazole (Sigma Cat# M2523), thiabendazole (Sigma Cat#T8904), oxfendazole (Sigma Cat#34176), fenbendazole (Sigma Cat# F5396) dissolved in DMSO as well as a DMSO negative control.
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| Contributor(s) |
Gilleard JS, Stasiuk SJ, Workentine ML |
| Citation(s) |
31542693 |
| Submission date |
Apr 29, 2019 |
| Last update date |
Oct 28, 2019 |
| Contact name |
John Stuart Gilleard |
| E-mail(s) |
jsgillea@ucalgary.ca
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| Phone |
403210 6327
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| Organization name |
University of Calgary
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| Department |
Faculty Veterinary Medicine
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| Street address |
3330, Hospital Dr, NW
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| City |
Calgary |
| State/province |
Alberta |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA540299 |
| SRA |
SRP194189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE130466_count_data.tsv.gz |
666.6 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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