|
| Status |
Public on Aug 27, 2019 |
| Title |
Single-cell transcriptomic analysis of the scrib mutant wing imaginal discs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Investigation of intratumor heterogeneity in the scrib¹ mutant wing imaginal discs.
Method: Staged scrib¹ wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina platform were performed by Novogene.
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| Overall design |
10X based single-cell mRNA profiles of 4, 5, 8, 14-day scrib mutant wing imaginal discs from prolonged larval stages.
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| Contributor(s) |
Zhang L, Ying W, Deng M, Yan Y |
| Citation(s) |
31371383, 31455604 |
| Submission date |
May 01, 2019 |
| Last update date |
Dec 03, 2019 |
| Contact name |
Yan Yan |
| E-mail(s) |
drosophilaust@gmail.com
|
| Organization name |
HKUST
|
| Department |
LIFS
|
| Street address |
Clear Water Bay
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA540653 |
| SRA |
SRP194385 |
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