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Series GSE13068 Query DataSets for GSE13068
Status Public on Oct 08, 2008
Title Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish
Organism Danio rerio
Experiment type Expression profiling by array
Summary Investigating neuronal and photoreceptor regeneration in the retina of zebrafish has begun to yield insights into both the cellular and molecular means by which this lower vertebrate is able to repair its central nervous system. However, knowledge about the signaling molecules in the local microenvironment of a retinal injury and the transcriptional events they activate during neuronal death and regeneration is still lacking. To identify genes involved in photoreceptor regeneration, we combined light-induced photoreceptor lesions, laser-capture microdissection (LCM) of the outer nuclear layer (ONL) and analysis of gene expression to characterize transcriptional changes for cells in the ONL as photoreceptors die and are regenerated. Using this approach, we were able to characterize aspects of the molecular signature of injured and dying photoreceptors, cone photoreceptor progenitors and microglia within the ONL. We validated changes in gene expression and characterized the cellular expression for three novel, extracellular signaling molecules that we hypothesize are involved in regulating regenerative events in the retina.

 
Overall design As a means to identify genes necessary for photoreceptor regeneration, we evaluated transcriptional changes for cells in the outer nuclear layer (ONL) as photoreceptors die and are regenerated. To accomplish this, we combined light-induced photoreceptor lesions, laser-capture microdissection (LCM) of the ONL and analysis of gene expression using oligonucleotide arrays.
 
Contributor(s) Craig SE, Calinescu A, Hitchcock PF
Citation(s) 20072637
Submission date Oct 06, 2008
Last update date Jan 25, 2018
Contact name Peter F. Hitchcock
E-mail(s) peterh@umich.edu
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (15)
GSM327232 control retina T0-sample5
GSM327233 control retina T0-sample6
GSM327234 control retina T0-sample7
Relations
BioProject PRJNA109685

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13068_RAW.tar 27.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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