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Series GSE130767 Query DataSets for GSE130767
Status Public on Apr 22, 2020
Title miRNA profiling of cultured primary recessive dystrophic epidermolysis bullosa (RDEB) squamous cell carcinoma (SCC)
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Recessive dystrophic epidermolysis bullosa (RDEB) is a monogenetic skin disorder caused by mutations in the COL7A1 gene. Missing type VII collagen leads to severe blister formation and frequent chronic wounds. Patients suffering from RDEB are prone to develop particulary aggressive squamous cell carcinoma (SCC), representing the major cause of mortality. This dataset provides Affymetrix microarray (miRNA4.1) based whole transcriptome data on RNA isolated from cultured primary keratinocytes (KC) as well as squamous cell carcinoma (SCC).
Cells were derived from punch biopsies or tumor resections from either healthy donors or SCC patients with or without the diagnosis recessive dystrophic epidermolysis bullosa (RDEB). Primary KC and SCC were cultivated in fully defined medium till subconfluency. Total RNA was isolated and microarray assay performed.
 
Overall design A case-control study design of cultured primary cells representing RDEB-SCC, RDEB-KC, non-EB SCC, healthy control KC.
 
Contributor(s) Wimmer M, Zauner R, Wally V
Citation(s) 32276641
Submission date May 06, 2019
Last update date Apr 30, 2021
Contact name Roland Zauner
E-mail(s) rolan.zauner@salk.at
Organization name EB House Austria
Department University Hospital of Dermatology Salzburger Landeskliniken (SALK) | Paracelsus Medical University Salzburg (PMU)
Lab small molecules & epigenetics
Street address Muellner Hauptstrasse 48
City Salzburg
State/province Salzburg
ZIP/Postal code 5020
Country Austria
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (20)
GSM3753293 HC-KC_01
GSM3753294 HC-KC_02
GSM3753295 HC-KC_03
Relations
BioProject PRJNA541300

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Supplementary file Size Download File type/resource
GSE130767_RAW.tar 17.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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