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| Status |
Public on Jun 01, 2019 |
| Title |
To investigate whether recover DNMT3A expression permitted adult beta cell of βRapKO establishing proper transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Murine islets were isolated from 8-week-old WT and βRapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80°C for preparation for RNA sequencing.
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| Overall design |
Transcriptome of three groups: WT, βRapKO, βRapKO+LvDnmt3a
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| Contributor(s) |
Ni Q, Wang Q, Ning G |
| Citation missing |
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| Submission date |
May 08, 2019 |
| Last update date |
Jun 01, 2019 |
| Contact name |
Qicheng Ni |
| E-mail(s) |
niqicheng8@qq.com
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| Phone |
+8615216616283
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| Organization name |
Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine
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| Street address |
197 Ruijin 2nd Road
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA541937 |
| SRA |
SRP197113 |
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