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| Status |
Public on Jun 10, 2020 |
| Title |
Transcriptomic analysis of bone and fibrous tissue morphogenesis during digit tip regeneration in the adult mouse |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Investigate the transcriptomic landscape throughout the time course of murine digit regeneration after level-dependent amputation.
Methods: The terminal phalanx bone of hind limb digits was subjected to either a distal amputation (25% bone length loss) or proximal amputation (65% bone length loss). Total RNA was isolated from bone and fibrous tissues distal to the distal interphalangeal joint at 12, 14, and 21 days post-amputation (DPA). mRNA library was sequenced using Illumina HiSeq 3000. Trimmed reads were aligned to the mouse genome mm10 and batch-corrected.
Results: A limb-specific developmental signaling pathway is transiently upregulated at 14 DPA after distal amputation, corresponding with regeneration of digit tip tissues. Absence of a limb-specific pathway after proximal amputation corresponded with minimal regeneration and fibrotic scarring.
Conclusions: Digit regeneration is a level-dependent and spatiotemporally controlled process, with distal and proximal amputations showing significant differences in gene expression and tissue regrowth over time.
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| Overall design |
mRNA profiles of the murine digit tip at various time points after distal amputation or proximal amputation were generated using high throughput sequencing.
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| Contributor(s) |
Qu F, Palte IC, Gontarz PM, Zhang B, Guilak F |
| Citation(s) |
32506623 |
| Submission date |
May 13, 2019 |
| Last update date |
Jun 10, 2020 |
| Contact name |
Bo Zhang |
| E-mail(s) |
bzhang29@wustl.edu
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| Phone |
3143624757
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| Organization name |
Washington University School of Medicine
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| Department |
Developmental Biology
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| Lab |
Zhang
|
| Street address |
4515 McKinley Research Bldg 03212
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA542610 |
| SRA |
SRP198228 |
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