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| Status |
Public on Oct 11, 2008 |
| Title |
Identification of PU.1 target genes by expression profiling of PUER cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
PU.1 is a key transcription factor for macrophage differentiation. Novel PU.1 target genes were identified by mRNA profiling of PU.1-deficient progenitor cells (PUER) before and after PU.1 activation. We used two different types of Affymetrix DNA-microarrays (430 2.0 arrays and ST 1.0 exon arrays) to characterize the global PU.1-regulated transcriptional program underlying the early processes of macrophage differentiation.
Keywords: time course
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| Overall design |
Uninduced and 24h 4-hydroxy-tamoxifen (OHT) induced PUER cells were cultured before RNA extraction and hybridization on Affymetrix Mouse Genome 430 2.0 and Mouse Exon ST 1.0 microarrays. Triplicates before (0h) and after (24h) induction of PU.1 were analyzed.
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| Contributor(s) |
Langmann T, Weigelt K |
| Citation(s) |
19167354, 20709948 |
| Submission date |
Oct 08, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Thomas Langmann |
| E-mail(s) |
thomas.langmann@klinik.uni-regensburg.de
|
| Phone |
+49-941-944-5423
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| Fax |
+49-941-944-5402
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| Organization name |
Institute of Human Genetics
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| Department |
University of Regensburg
|
| Street address |
Franz-Josef-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA109667 |
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