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Series GSE131280 Query DataSets for GSE131280
Status Public on May 16, 2019
Title DNA Methylation of primary human fibroblasts across replicative lifespan in normal and hyperglycemic conditions
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Genome wide DNA methylation profiling of normal (5.5mM) and high (25mM) glucose samples from primary human fibroblasts across the cellular lifespan. The Illumina Infinium EPIC Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 866,836 CpGs. Samples included 12 normal glucose time points and 14 high glucose time points, as well as, 6 technical replicates of normal glucose timepoint at 34.8 days. Methylation data was used to estimate the rate of aging occuring across replicative lifespan using several DNA methylation based Aging Clocks. Other analysis include comparing the overlap of age-related CpGs in vitro to in vivo, and describing the diverse kinetics of methylation sites across cellular lifespan.
 
Overall design Bisulphite converted DNA from the 31 samples were hybridised to the Illumina Infinium EPIC Human Methylation Beadchip v1.0
 
Contributor(s) Sturm G, Cardenas A, Bind M, Horvath S, Wang S, Wang Y, Hägg S, Hirano M, Picard M
Citation(s) 31156022
Submission date May 15, 2019
Last update date Aug 15, 2019
Contact name Gabriel Sturm
E-mail(s) gs2934@cumc.columbia.edu
Organization name Columbia University
Department Psychiatry
Lab Picard Lab
Street address 40 Haven Avenue
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (31)
GSM3768585 hFB_NG_35days_rep1
GSM3768586 hFB_NG_35days_rep2
GSM3768587 hFB_NG_35days_rep3
Relations
BioProject PRJNA543113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131280_RAW.tar 593.8 Mb (http)(custom) TAR (of IDAT)
Processed data included within Sample table

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