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| Status |
Public on Oct 31, 2019 |
| Title |
Analysis of gene expression in CNOT2-depleted A549 lung cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
CNOT2 depletion partially decreased number of colony in colony forming assay and cell number in proliferation assay for NSCLC cells. Co-treatment with TRAIL and depletion of CNOT2 dramatically declined survival rate and increased apoptosis compared with TRAIL only in TRAIL resistant NSCLC cells. So, to find major signaling molecules in CNOT2 depleted condition of A549 cells, gene expression analysis was designed.
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| Overall design |
NSCLC cells were plated at 60% confluency in 60mm dish 24 hours before transfection. shRNAs were designed against specific target sequence of human CNOT2 mRNAs.
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| Contributor(s) |
Yun M, Kim S |
| Citation(s) |
31894259 |
| Submission date |
May 20, 2019 |
| Last update date |
Feb 10, 2020 |
| Contact name |
Miyong Yun |
| E-mail(s) |
myyun91@gmail.com
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| Phone |
82-10-9269-3086
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| Organization name |
Sejong University
|
| Department |
Bioindustry and bioresource engineering
|
| Street address |
209, Neungdong-ro, Gwangjin-gu
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| City |
Seoul |
| ZIP/Postal code |
05006 |
| Country |
South Korea |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA543868 |
