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Series GSE13157 Query DataSets for GSE13157
Status Public on Oct 16, 2008
Title The role of ERbeta2 in zebrafish neuromasts development 15uM
Organism Danio rerio
Experiment type Expression profiling by array
Summary The role of ERbeta2 in zebrafish larvae was investigated by injection of a Morpholino against ERbeta2. After 72hpf, the morphants showed a strong disruption in their sensory systems. ERbeta2 has been shown to be needed for the normal functioning of the sensory system organs, the neuromasts. The mechanisms involved in the neuromast disruption in ERbeta2 morphants was identified by microarrays gene screening. After comparison of two screening with low and high concentration of Morpholinos, genes that were present in the two microarrays screening were selected. The genes were then chosen by relevance for the mechanisms involved in the role of ERbeta2 in neuromast development. The ngn1 transcription factor, Notch3 and Notch1a showed to be up-regulated, also confirmed by in situ hybridization. The Notch signaling is known to be involved in cell fate in developing neuromasts. The overall conclusion is that ERbeta2 by interacting with the notch signaling pathways is critical for normal development of the neuromast of the lateral line in zebrafish.

Keywords: knock down experiment
 
Overall design Zebrafish eggs until 4 cell stage were injected either with Morpholino blocking the translation ERbeta2 (ERbeta2 MO), either with a standard control Morpholino (coMO) at a concentration of 15uM. Two biological replicates for ERbeta2 MO, two replicates for coMO and two replicates for uninjected embryos (blank). A total of 6 samples were performed.
 
Contributor(s) Fröhlicher M
Citation(s) 19289112
Submission date Oct 10, 2008
Last update date Jan 25, 2018
Contact name Mirjam Föhlicher
E-mail(s) mirjam.froehlicher@eawag.ch
Organization name eawag
Street address überlandstrasse 133
City Dübendorf
ZIP/Postal code 8600
Country Switzerland
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM329381 Embryos injected with 15uM ERbeta2 MO biological repl. 1
GSM329382 Embryos injected with 15uM ERbeta2 MO biological repl. 2
GSM329383 Embryos injected with 15uM coMO biological repl. 1
This SubSeries is part of SuperSeries:
GSE13771 The role of ERbeta2 in zebrafish neuromasts development
Relations
BioProject PRJNA114107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13157_15uM_ERbeta2MO_blank.txt 128.8 Kb (ftp)(http) TXT
GSE13157_15uM_ERbeta2MO_coMO.txt 55.6 Kb (ftp)(http) TXT
GSE13157_15uM_blank-coMO.txt 28.0 Kb (ftp)(http) TXT
GSE13157_RAW.tar 13.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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