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Status |
Public on Oct 28, 2020 |
Title |
Bisulfite-free epigenomics and genomics of single cells analyzed by methylation sensitive restriction (epi-gSCAR) |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by high throughput sequencing Other
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Summary |
Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between two cell lines based on the DNA methylation and genetic profiles.
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Overall design |
DNA methylation and gene variant analysis of 27 single Kasumi-1 and 20 single OCI-AML3 AML cells
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Contributor(s) |
Niemöller C, Wehrle J, Becker H |
Citation(s) |
33526904 |
Submission date |
May 23, 2019 |
Last update date |
Feb 03, 2021 |
Contact name |
Heiko Becker |
Organization name |
University Medical Center
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Department |
Department of Medicine I
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Lab |
Becker
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Street address |
Breisacher Straße 115
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City |
Freiburg |
State/province |
Baden-Württemberg |
ZIP/Postal code |
79106 |
Country |
Germany |
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Platforms (2) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (49)
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Relations |
BioProject |
PRJNA544519 |
SRA |
SRP199364 |