|
| Status |
Public on Jun 09, 2020 |
| Title |
Quality control measures for placental sample purity in DNA methylation array analyses |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
The purity of tissue samples can affect the accuracy and utility of DNA methylation array analyses. This is particularly important for the placenta which is hypomethylated. Placental villous tissue from early pregnancy terminations can be difficult to separate from the non-villous tissue, resulting in potentially inaccurate results. We used several methods to identify mixed placental samples using DNA methylation array datasets from our laboratory and those contained in the NCBI GEO database, highlighting the importance of determining sample purity during quality control processes.
|
| |
|
| Overall design |
Placental samples from 10 first trimester placentas (chorionic villi) were profiled using the Infinium HumanMethylation850 BeadChip.
|
| |
|
| Contributor(s) |
Wan Q, Roberts CT |
| Citation(s) |
31569011 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD089685 |
Maternal molecular profiles reflect placental function and development across gestation |
THE UNIVERSITY OF ADELAIDE |
Claire Roberts |
|
| Submission date |
May 30, 2019 |
| Last update date |
Jun 09, 2020 |
| Contact name |
Claire Roberts |
| E-mail(s) |
claire.roberts@adelaide.edu.au
|
| Organization name |
The University of Adelaide
|
| Department |
Adelaide Medical School
|
| Street address |
AHMS building
|
| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platforms (1) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA545414 |
Less...
More...