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| Status |
Public on Jun 30, 2019 |
| Title |
Methylation in Adaptive Natural Killer cells after exposure to NKG2C agonist antibodies for 7 days |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Natural Killer cells from cytomegalovirus positive individuals (i.e. adaptive NK) where cultured for seven days with either control antibody, an NKG2C agonist antibody, or an antibody agonist to NKG2C and NKG2A
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| Overall design |
NK cells were isolated from peripheral blood by Ficoll-Paque and then a Miltenyi bead kit. They were cultured for seven days in B0 media with 10 ng/mL IL-15 and platebound isotype antibody, anti-NKG2C, or anti-NKG2A. After seven days cells were pelleted and DNA extracted for bisulfite sequencing and analysis by Ilumina Infinium methylationEPIC Beadchips
NA10858_batch 2 (control nucleic acid) and NA10858_batch 1 (control nucleic acid) should not have beta values as they are controls, not samples. We did not generate Beta values for samples 1215 (A,B,C) and 1233 (A,B,C) because the raw data did not have good quality control across the three experimental conditions. Therefore we did not use these for analysis for our paper but submitted the raw data for transparency sake.
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| Contributor(s) |
Merino A, Zhang B, Dougherty P, Wang J, Blazar B, Miller J, Cichocki F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 30, 2019 |
| Last update date |
Jul 02, 2019 |
| Contact name |
Aimee Merino |
| E-mail(s) |
merin008@umn.edu
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| Phone |
6512005813
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| Organization name |
University of Minnesota
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| Lab |
Aimee Merino
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| Street address |
420 Delaware Street SE
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| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA545485 |
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