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| Status |
Public on Apr 08, 2020 |
| Title |
Acquired resistance to combined BET and CDK4/6 inhibition in triple-negative breast cancer [Exome] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome variation profiling by high throughput sequencing
|
| Summary |
BET inhibitors are promising therapeutic agents for the treatment of triple-negative breast cancer (TNBC), but the rapid emergence of resistance necessitates investigation of combination therapies and their effects on tumor evolution. Here, we show that palbociclib, a CDK4/6 inhibitor, and paclitaxel, a microtubule inhibitor, synergize with the BET inhibitor JQ1 in TNBC lines. High-complexity DNA barcoding and mathematical modeling indicate a high rate of de novo acquired resistance to these drugs relative to pre-existing resistance. We demonstrate that the combination of JQ1 and palbociclib induces cell division errors, which can increase the chance of developing aneuploidy. Characterizing acquired resistance to combination treatment at single cell level shows heterogeneous mechanisms including activation of G1-S and senescence pathways. Our results establish a rationale for further investigation of combined BET and CDK4/6 inhibition in TNBC and suggest novel mechanisms of action for these drugs, and new vulnerabilities in cells after emergence of resistance.
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| |
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| Overall design |
Exome-seq of SUM159 breast cancer cell line treated in vitro with JQ1, palbociclib, paclitaxel, and combinations
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| |
|
| Contributor(s) |
Ge JY, Michor F, Polyak K |
| Citation(s) |
32393766 |
| Submission date |
May 30, 2019 |
| Last update date |
May 19, 2020 |
| Contact name |
Kornelia Polyak |
| E-mail(s) |
kornelia_polyak@dfci.harvard.edu
|
| Phone |
617-632-2106
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
Polyak
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (23)
|
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| This SubSeries is part of SuperSeries: |
| GSE131986 |
Acquired resistance to combined BET and CDK4/6 inhibition in triple-negative breast cancer |
|
| Relations |
| BioProject |
PRJNA545506 |
| SRA |
SRP199905 |
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