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| Status |
Public on Oct 15, 2019 |
| Title |
Genome-wide maps of chromatin accessibility before and after NR2F2 knock down using ATAC-seq. |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
FOXA1 and GATA3 can remodel chromatin accessibility, we further explored what effect of NR2F2 would have on chromatin properties. ATAC-seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing) is widely used to map chromatin accessibility genome-wide. Thus, We perform ATAC-seq without oestrogen stimulation before and after NR2F2 depletion.Covalent modifications are a main chromatin property.To test whether NR2F2 favoured histone modification deposition on chromatin, we profiled ChIP-Seq of H3K4me1, H3K4me3, and H3K27ac following NR2F2 depletion in oestrogen-starved MCF-7 cells to gain comprehensive histone medication landscape.
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| Overall design |
Study of chromatin accessibility in WT and NR2F2 KO MCF-7 cells by deep sequencing using Illumina xten.
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| Contributor(s) |
Jiang G, Wang X |
| Citation(s) |
31588232 |
| Submission date |
Jun 10, 2019 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Guojuan Jiang |
| E-mail(s) |
jgj5292@126.com
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| Phone |
18817821244
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| Organization name |
Shanghai Jiaotong University
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| Department |
School of Medicine
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| Lab |
State Key Laboratory of Medical Genomics
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| Street address |
Ruijin er road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA548081 |
| SRA |
SRP200961 |
