NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE13247 Query DataSets for GSE13247
Status Public on Nov 06, 2008
Title A role for mammalian Sin3 in permanent gene silencing
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary The multi-subunit Sin3 co-repressor complex regulates gene transcription through deacetylation of nucleosomes. However, the full range of Sin3 activities and targets is not well understood. Here, we have investigated genome-wide binding of mouse Sin3 and RBP2 as well as histone modifications and nucleosome positioning as a function of myogenic differentiation. Remarkably, we find that Sin3 complexes spread immediately downstream of the transcription start site on repressed and transcribed genes during differentiation. We show that RBP2 is part of a Sin3 complex, and on a subset of E2F4 target genes, the coordinated activity of Sin3 and RBP2 leads to deacetylation, demethylation, and repositioning of nucleosomes. Our work provides evidence for coordinated binding of Sin3, chromatin modifications, and chromatin remodeling within discrete regulatory regions, suggesting a model in which spreading of Sin3 binding is ultimately linked to permanent gene silencing on a subset of E2F4 target genes.
 
Overall design Factor binding on genome-wide promoter arrays were performed at least in duplicate. Factor binding on high density tiling arrays were performed at least in triplicate. Histone modifications and nucleosome density profiles were performed at least in duplicate
 
Contributor(s) van Oevelen C, Wang J, Asp P, Kluger Y, Yan Q, Kaelin WG Jr, Dynlacht BD
Citation(s) 18995834
Submission date Oct 16, 2008
Last update date May 04, 2018
Contact name Brian Dynlacht
E-mail(s) Brian.Dynlacht@nyumc.org
Phone 212-263-6162
Organization name NYU Medical Center
Department Cancer Institute
Lab Dynlacht
Street address 550 1 Av
City New York
State/province NY
ZIP/Postal code 10016
Country USA
 
Platforms (4)
GPL4128 Agilent-014716 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 (G4490A) (Feature Number version)
GPL4129 Agilent-014717 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 (G4490A) (Feature Number version)
GPL7495 Agilent-015963 Mouse Promoter tiling array
Samples (40)
GSM333366 differentiated C2C12 cells Sin3a binding chip on chip replicate 1
GSM333379 differentiated C2C12 cells Sin3a binding chip on chip replicate 1
GSM333459 differentiated C2C12 cells Sin3a binding chip on chip replicate 2
Relations
BioProject PRJNA109527

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13247_RAW.tar 2.5 Gb (http)(custom) TAR (of CEL, TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap