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| Status |
Public on Oct 23, 2008 |
| Title |
Gene expression profiles in rat lung after inhalation exposure to C60 fullerene or ultrafine NiO particles |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling of the rat lung after whole-body inhalation exposure to C60 fullerene and ultrafine nickel oxide (Uf-NiO) particles as a positive control were employed to gain insights into these molecular events.
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| Overall design |
Groups of 9-week-old male Wistar rats (n = 5 per group/time point) were exposed to clean air (negative control), C60 fullerene or Uf-NiO (positive control) suspension in a whole-body exposure chamber. Rats were exposed to aerosol for 6 hours a day, for 4 weeks (5 days a week). Standard chow diet was provided ad libitum during non-exposure hours. After exposure period for 4 weeks, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days and 1month (33 days) post-exposure. Lungs of anesthetized rats were perfused with physiological saline, excised, and used for C60 fullerene or nickel burdens in lungs (n = 4, left lungs), TEM analysis (n = 1, whole lungs) and microarray analysis (n = 4, right lungs).
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| Contributor(s) |
Fujita K, Iwahashi H |
| Citation(s) |
19167457 |
| Submission date |
Oct 17, 2008 |
| Last update date |
Sep 13, 2014 |
| Contact name |
Katsuhide Fujita |
| E-mail(s) |
ka-fujita@aist.go.jp
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| Organization name |
National Institute of Advanced Industrial Science and Technology
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| Department |
Research Institute of Science for Safety and Sustainability
|
| Street address |
Onogawa 16-1
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| City |
Tsukuba |
| ZIP/Postal code |
3058569 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA109529 |
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