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Series GSE132513 Query DataSets for GSE132513
Status Public on Jan 01, 2020
Title Epigenetic remodelling of enhancers in response to estrogen deprivation and re-stimulation [methylArray]
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER) orchestrates a complex molecular circuitry that is not yet fully elucidated. Here we investigated genome-wide DNA methylation, histone acetylation and transcription after estradiol (E2) deprivation and re-stimulation to better characterise the ability of ER to coordinate gene regulation. We found that E2 deprivation mostly resulted in DNA hypermethylation and histone deacetylation in enhancers. Transcriptome analysis revealed that E2 deprivation leads to a global down-regulation in gene expression. Enrichment analysis of transcription factor (TF) binding and motif occurrence in the proximity of E2 deprivation-mediated differentially methylated and acetylated sites reinforces the importance of AP-1 and FOX proteins, Finally, most deprivation-dependent epigenetic changes were reversed following E2 re-stimulation.
 
Overall design Control MCF-7 HTB-22 breast cancer cells (CTR) were cultured continuously for 14 days in E2-containing medium, while E2-deprived cells (E2D) were cultured in the same conditions as CTR only lacking E2. The re-stimulated cells (ReSt) were E2-deprived for 4 days and re-stimulated for the 10 following. Each treatment (CTR, E2D and ReSt) and time point (d0, d4, d14) is available in triplicates. See publication for schematic design
 
Contributor(s) Sklias A, Herceg Z
Citation(s) 34403459
Submission date Jun 11, 2019
Last update date Oct 06, 2021
Contact name Zdenko Herceg
E-mail(s) HercegZ@iarc.fr
Organization name IARC
Department EGM
Lab Epigenomics and mecanisms
Street address 150 Cours Albert Thomas
City Lyon
ZIP/Postal code 69372 CEDEX 08
Country France
 
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (18)
GSM3872480 CTR_D0_1
GSM3872481 E2D_D4_1
GSM3872482 Rest_D14_1
This SubSeries is part of SuperSeries:
GSE132514 Epigenetic remodelling of enhancers in response to estrogen deprivation and re-stimulation
Relations
BioProject PRJNA548421

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132513_RAW.tar 416.0 Mb (http)(custom) TAR (of IDAT)
GSE132513_matrix_signal_intensities.csv.gz 85.6 Mb (ftp)(http) CSV
Processed data included within Sample table

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