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Series GSE132519 Query DataSets for GSE132519
Status Public on Feb 18, 2020
Title High-throughput RNA sequencing analysis of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to evaluate the effects of si-LINC00152 on the mRNA of human colon cancer cell line HCT116.
Methods: Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA to cells or transfected with siRNA targeting LINC00152 for 36 hours in DMEM medium (with 10% serum). Total RNA were extracted and detected by Illumina high-throughput RNA sequencing data analysis. 2 independent biological replicates were plated, transfected in parallel for each control and siRNA.
Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. There are 90 mRNAs were up-regulated and 159 were down-regulated in “si-LINC00152” group comparing to “control” group.
Conclusions: Our study describes the mRNA changes of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.
 
Overall design Human colon cancer cell line HCT116 mRNA profiles of “control” (cells was transfected with a control non-targeting siRNA) and“si-LINC00152”(cells was transfected with LINC00152 siRNA) were generated by RNA sequecning, using Solexa high-throughput sequencing service (Oebiotech, Shanghai, China).
 
Contributor(s) Ou C, Ma J
Citation(s) 32042551
Submission date Jun 11, 2019
Last update date Apr 20, 2022
Contact name Jian Ma
E-mail(s) majian@csu.edu.cn
Organization name Central South University
Department Cancer Research Institute
Street address 110 Xiangya Road
City Changsha
State/province Hunan
ZIP/Postal code 410078
Country China
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (4)
GSM3872544 Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA, biological rep 1
GSM3872545 Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA, biological rep 2
GSM3872546 Human colon cancer cell line HCT116 was transfected with LINC00152 siRNA, biological rep 1
Relations
BioProject PRJNA548279
SRA SRP201071

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Supplementary file Size Download File type/resource
GSE132519_RAW.tar 470.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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