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Series GSE13255 Query DataSets for GSE13255
Status Public on Dec 01, 2009
Title Gene Expression Profiles in Peripheral Blood Mononuclear Cells Can Distinguish Patients with NonSmall Cell Lung Cancer.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We report a 29-gene diagnostic signature, which distinguishes individuals with NSCLC from controls with non-malignant lung disease with 91% Sensitivity, 79% Specificity and a ROC AUC of 92%. Accuracy on an independent set of 18 NSCLC samples from the same location was 79%. Samples from an independent location including 12 stage 1 NSCLC and 15 controls, achieved an accuracy of 74%. A study of 18 paired samples taken pre and post surgery shows that the PBMC associated “cancer” signature is significantly reduced after tumor removal, supporting the hypothesis that the signature detected in pre-surgery samples is a response to the presence of the tumor.
 
Overall design We compared microarray gene expression profiles in PBMC from patients with NSCLC and a control group with smoking-related non-malignant lung disease, a likely confounding factor. We found a distinguishing gene signature which was validated on 2 independent sets of hold-out samples one set from a different location. Gene expression changes were also compared between pre and post surgery samples from 18 patients.
 
Contributor(s) Showe MK, Vachani A, Kossenkov AV, Yousef M, Nichols C, Nickonova E, Alila L, Yie TA, Rom W, Albelda S, Showe LC
Citation(s) 19951989
Submission date Oct 17, 2008
Last update date Feb 15, 2013
Contact name Louise C Showe
E-mail(s) lshowe@wistar.org
Phone 215-898-3791
Organization name The Wistar Institute
Lab Dr. Louise Showe
Street address 3601 Spruce St
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL6102 Illumina human-6 v2.0 expression beadchip
Samples (291)
GSM334354 Case/control experiment: C.9.10001.187.QbF83.u.HTN
GSM334355 Case/control experiment: Cm.9.10003.201.SbF43.u.HTN
GSM334356 Case/control experiment: C.9.10004.194.QwM54.u.PS
Relations
BioProject PRJNA109539

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13255_RAW.tar 147.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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