|
| Status |
Public on Sep 30, 2019 |
| Title |
In vitro maturation of intestinal organoids differentiated from wild type and STAT3 knock out human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human intestinal organoids (hIOs) resemble the human intestine physiologically and structurally. We recently present an in vitro maturation technique for generating mature and functional hIOs from human pluripotent stem cells (hPSCs). Here, we investigated the mechanisms of STAT3 for inducing in vitro maturation of hIOs. Using CRISPR/Cas9-mediated gene editing, STAT3 knockout (KO) human embryonic stem cell (hESC) lines were generated and characterized. By genome-wide microarray analyses, STAT3 KO hIOs showed markedly different profiles from the in vitro matured hIOs and human small intestine and the majority of genes, which are associated with intestinal development and functions, were downregulated by STAT3 KO. This study reveals important signaling pathways for the maturation of hIOs derived from hPSCs.
|
| |
|
| Overall design |
Gene expression profile of hIOs differentiated from WT and STAT3 KO hESCs with or without in vitro maturation, and adult human small intestine (hSI).
|
| |
|
| Contributor(s) |
Son M, Jung K, Kwon O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 11, 2019 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Mi-Young Son |
| E-mail(s) |
myson@kribb.re.kr
|
| Phone |
82-42-860-4426
|
| Organization name |
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
|
| Street address |
111 Gwahangno, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (7)
|
| GSM3874698 |
Wild type hESCs-intestinal organoid |
| GSM3874699 |
Wild type hESCs-intestinal organoid + co-culture |
| GSM3874700 |
Wild type hESCs-intestinal organoid + IL-2 |
| GSM3874701 |
STAT3 knock out hESCs-intestinal organoid |
| GSM3874702 |
STAT3 knock out hESCs-intestinal organoid + co-culture |
| GSM3874703 |
STAT3 knock out hESCs-intestinal organoid + IL-2 |
| GSM3874704 |
Human small intestine |
|
| Relations |
| BioProject |
PRJNA548361 |
Less...
More...