|
| Status |
Public on Nov 10, 2019 |
| Title |
RNA-seq analysis after knockdown of ASCL1 gene in Kelly cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq analysis was performed in Kelly neuroblastoma cell line to analyze gene expression changes after ASCL1 knockdown.
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| |
|
| Overall design |
Lentiviral shRNA transduction was carried out in Kelly cells to knockdown control GFP or ASCL1. One shRNA was used to target each gene. Experiments were done in biological duplicates. Total RNA was harvested 3 days after knockdown and RNA-seq analysis was performed.
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| |
|
| Contributor(s) |
Wang L, Tan TK, Sanda T |
| Citation(s) |
31819055 |
| Submission date |
Jun 13, 2019 |
| Last update date |
Feb 09, 2020 |
| Contact name |
Tze King Tan |
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute
|
| Lab |
Takaomi Sanda Lab
|
| Street address |
14 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platforms (1) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE132760 |
ASCL1 is a MYCN- and LMO1-dependent member of the adrenergic neuroblastoma core regulatory circuitry |
|
| Relations |
| BioProject |
PRJNA548772 |
| SRA |
SRP201348 |
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