|
| Status |
Public on Oct 23, 2008 |
| Title |
Gene expression analysis of paediatric acute lymphoblastic leukaemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We addressed the clinical significance and mechanisms behind in vitro cellular responses to ionising radiation (IR)-induced DNA double strand breaks in 74 paediatric ALL patients. We found an apoptosis-resistant response in 36% of patients and an apoptosis-sensitive response in the remaining 64% of leukaemias. Global gene expression profiling of 11 apoptosis-resistant and 11 apoptosis-sensitive ALLs revealed abnormal up-regulation of multiple pro-survival pathways in response to IR in apoptosis-resistant leukaemias and differential post-transcriptional activation of the PI3-Akt pathway was observed in representative resistant cases. It is possible that abnormal pro-survival responses to DNA damage provide one of the mechanisms of primary resistance in ALL .
Keywords: Gene expression profiling; response to irradiation
|
| |
|
| Overall design |
Twenty two B-precursor ALL tumours (11 responsive to IR-induced DNA damage and 11 resistant) were analysed before and 8 hours after exposure to 5 Gy IR, using the Affymetrix HG_U133A (GPL96) platform.
|
| |
|
| Contributor(s) |
Stankovic T, Marston E, McConville C, Falciani F |
| Citation(s) |
18941120 |
| Submission date |
Oct 20, 2008 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Carmel M McConville |
| E-mail(s) |
c.mcconville@bham.ac.uk
|
| Organization name |
University of Birmingham
|
| Department |
School of Cancer Sciences
|
| Street address |
Vincent Drive
|
| City |
Birmingham |
| State/province |
UK |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (44)
|
|
| Relations |
| BioProject |
PRJNA109599 |
Less...
More...