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| Status |
Public on Aug 31, 2019 |
| Title |
Single-cell transcriptomic analysis of 96hour AEL wild type wing imaginal discs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Genotype: FRT82B
Method: Staged wild type wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene.
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| Overall design |
10X based single-cell mRNA profiles of wild type wing imaginal discs
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| Contributor(s) |
Zhang L, Ying W, Deng M, Yan Y |
| Citation(s) |
31455604 |
| Submission date |
Jun 24, 2019 |
| Last update date |
Dec 03, 2019 |
| Contact name |
Yan Yan |
| E-mail(s) |
drosophilaust@gmail.com
|
| Organization name |
HKUST
|
| Department |
LIFS
|
| Street address |
Clear Water Bay
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA550366 |
| SRA |
SRP211811 |
