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Status |
Public on Aug 10, 2010 |
Title |
Changes in gene expression induced by apple polyphenol (APP) |
Organism |
Bos taurus |
Experiment type |
Expression profiling by array
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Summary |
γδ T cells are lymphocytes that function in both innate and adaptive immune responses, and our laboratory has previously reported that both bovine and human γδ T cells are primed for secondary responses by tannin components of the unripe apple peel (APP). In this report we began to investigate the mechanism by which priming occurs. We performed a microarray analysis on sorted bovine γδ T cells treated with APP and observed significant increases in transcripts encoding select inflammatory cytokines, such as GM-CSF, IL-8, and IL-17, but not markers of TCR stimulation such as IFNγ. When injected into the gut of mice, APP induced a robust influx of neutrophils into both the blood and peritoneum as measured by FACS. Importantly, both GM-CSF and IL-8 proteins were detected in these animals. Further studies were performed using the human γδ T cell-line MOLT-14, as large numbers of cells were required for subsequent experiments. Using these cells we found that both the GM-CSF and IL-8 mRNAs were significantly upregulated and stabilized in cells treated with APP. Finally, we show that the ERK1/2 MAPK pathway was activated in APP-treated MOLT-14 cells, and that this pathway plays a role in the mRNA stabilization we observed. Together, our data describe a unique inflammatory response in bovine, murine, and human γδ T cells in response to APP, and suggest that mRNA stability mechanisms could account for the priming phenotype we previously observed.APP has a distinctive gamma delta T cells specific priming activity.
Keywords: comparison of 2 treatment types, tannin, gamma delta T cells
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Overall design |
To begin to understand the effects of APP in innate immunity, we investigated the global gene expression profiles of stimulated bovine gamma delta T cells. Peripheral blood from 2 neonatal bovine calves was collected. gamma delta T cells were sorted to >97% purity using a FACS Vantage. Cells were placed in culture and stimulated with either an aqueous extract of APP (42.2ug/ml) or PBS for 4 hours after which RNA was extracted and processed for microarray analysis following standard protocols from Affymetrix.
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Contributor(s) |
Hedges J, Daughenbaugh K |
Citation missing |
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Submission date |
Oct 23, 2008 |
Last update date |
Dec 17, 2012 |
Contact name |
Jodi Fern Hedges |
E-mail(s) |
jodi.hedges@gmail.com
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Phone |
406-994-6384
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Organization name |
Montana State University
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Department |
Veterinary Molecular Biology
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Lab |
Jutila
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Street address |
960 Technology Blvd.
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City |
Bozeman |
State/province |
MT |
ZIP/Postal code |
59718 |
Country |
USA |
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Platforms (1) |
GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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Samples (4)
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GSM336327 |
gd T cells from calf 301 stimulated with vehicle only control. |
GSM336328 |
gd T cells from calf 301 stimulated with apple polyphenol (APP). |
GSM336329 |
gd T cells from calf 303 stimulated with vehicle only control. |
GSM336330 |
gd T cells from calf 303 stimulated with apple polyphenol (APP). |
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Relations |
BioProject |
PRJNA109911 |
Supplementary file |
Size |
Download |
File type/resource |
GSE13321_RAW.tar |
8.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
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