|
| Status |
Public on Sep 05, 2020 |
| Title |
The effect of YAP/TAZ on the expression of the heat shock transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We found that heat stress induces a strong and rapid YAP dephosphorylation and activation. To determin what is the physiological functions of YAP in response to heat stress, we performed RNA-SEQ analysis on siCon and siYAP/TAZ knockdown tumor cells with or without heat shock. Results indicated that 95% of heat inducible genes is blocked (83% completely and 12% partially) by YAP/TAZ knockdown in the B16-OVA melanoma cells whereas only 5% of heat inducible genes were unaffected, supporting a previously unrecognized prominent role of YAP/TAZ in the expression of the heat shock transcriptome and cell survival.
|
| |
|
| Overall design |
siCon and siYAP/TAZ knockdown cells with or without heat shock, in triplicate.
|
| |
|
| Contributor(s) |
Luo M, Shao B |
| Citation(s) |
33199845 |
| Submission date |
Jun 24, 2019 |
| Last update date |
Jan 05, 2021 |
| Contact name |
Min Luo |
| E-mail(s) |
minluo0803@scu.edu.cn
|
| Organization name |
SICHUAN UNIVERSITY
|
| Street address |
NO.17, the third section of South Renmin Road
|
| City |
CHENGDU |
| State/province |
SICHUAN |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA551001 |
| SRA |
SRP212072 |
Less...
More...