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| Status |
Public on Sep 24, 2019 |
| Title |
RNAseq data from mtDNA mutator induced pluripotent stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNAsequencing data from POLG D257A mutant mouse iPS (induced pluripotent stem) cells. These mice accumulate random point mutations in their mitochondrial genome and manifest progeric features. The reprogramming and phenotype of the cells is described in Hämäläinen et al. 2015, Cell reports, mtDNA Mutagenesis Disrupts Pluripotent Stem Cell Function by Altering Redox Signaling. The goal of the study was to analyze altered gene expression profiles between mutant and wt iPS cells to identify altered biological pathways responsible for the stemness and proliferation defects seem in mtDNA mutator stem cells.
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| Overall design |
The sample set includes 5 independent mutator iPSC lines, homozygous for the POLG D257A mutation, and 3 wt control lines derived from littermate control embryos.
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| Contributor(s) |
Suomalainen A, Hämäläinen RH |
| Citation |
Hamalainen, R.H., Landoni, J.C., Ahlqvist, K.J. et al. Defects in mtDNA replication challenge nuclear genome stability through nucleotide depletion and provide a unifying mechanism for mouse progerias. Nat Metab 1, 958 965 (2019) doi:10.1038/s42255-019-0120-1
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| Submission date |
Jun 25, 2019 |
| Last update date |
Jan 02, 2020 |
| Contact name |
Riikka H Hämäläinen |
| E-mail(s) |
riikka.martikainen@uef.fi
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| Organization name |
university of eastern Finland
|
| Street address |
Neulaniementie 2
|
| City |
Kuopio |
| ZIP/Postal code |
70210 |
| Country |
Finland |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA550531 |
| SRA |
SRP212050 |
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