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| Status |
Public on Jun 26, 2019 |
| Title |
Expression data from rosmarinic acid-treated human amnion epithelial cells (hAECs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals a potential role of rosmarinic acid in neural induction and neuronal differentiation of hAECs.
hAECs were isolated from discarded term placenta and were treated with 20 μM RA for seven days. Microarray gene expression profiling was conducted for biological replicates of RA-treated (T7) and untreated control cells on day 0 (D0) and day 7 (D7).
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). Two hundred and fifty ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.
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| Citation(s) |
31396047, 35433672 |
| Submission date |
Jun 25, 2019 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Farhana Ferdousi |
| E-mail(s) |
farhana_ferdousi24@yahoo.com
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| Organization name |
University of Tsukuba
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| Street address |
Tennodai 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8575 |
| Country |
Japan |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA550970 |
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